A Simple, Rapid, Inexpensive Assay for Toxic Chemicals Using a Bacterial Indicator

نویسندگان

  • J. L. Botsford
  • T. Hillaker
  • B. Robertson
  • M. Gonzales
  • M. Benavidez
  • B. Jones
  • R. Baker
  • W. Steen
  • F. Pacheco
  • V. Homer
  • O. Lucero
  • M. Matthews
چکیده

A simple test for toxic chemicals has been developed. Rhizobium meliloti is combined with the toxic chemical. A tetrazolium dye, MTT (3-[4,5-Dimethylthiazol-2-yl]2,5diphenyl-tetrazolium bromide) is added. The bacterium reduces this dye, causing the optical absorbance to increase dramatically. The increase can be determined with a simple spectrophotometer. Toxic chemicals and minerals inhibit the reduction of the dye. Presumably the dye serves as a terminal electron acceptor for electron transport. Toxic substances presumably damage the electron transport system. The results compare favorably with published results of tests using the MicrotoxTM assay and with the PolytoxTM assay. This assay is simpler and requires no specialized equipment. It should be possible to use this assay in a third world situation.

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A Simple, Inexpensive, and Rapid Method to Determine Toxicity Using a Bacterial Indicator Organism

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تاریخ انتشار 1996