A Simple, Rapid, Inexpensive Assay for Toxic Chemicals Using a Bacterial Indicator
نویسندگان
چکیده
A simple test for toxic chemicals has been developed. Rhizobium meliloti is combined with the toxic chemical. A tetrazolium dye, MTT (3-[4,5-Dimethylthiazol-2-yl]2,5diphenyl-tetrazolium bromide) is added. The bacterium reduces this dye, causing the optical absorbance to increase dramatically. The increase can be determined with a simple spectrophotometer. Toxic chemicals and minerals inhibit the reduction of the dye. Presumably the dye serves as a terminal electron acceptor for electron transport. Toxic substances presumably damage the electron transport system. The results compare favorably with published results of tests using the MicrotoxTM assay and with the PolytoxTM assay. This assay is simpler and requires no specialized equipment. It should be possible to use this assay in a third world situation.
منابع مشابه
A Simple, Inexpensive, and Rapid Method to Determine Toxicity Using a Bacterial Indicator Organism
A simple, inexpensive and rapid method to determine the toxicity of compounds, isolated chemicals, soil samples, and water samples has been developed. The test uses the bacterium, Rhizobium meliloti, as the indicator organism. Toxicity values obtained with this test are complarable to those obtained with more familiar tests like the sand flea Daphnia magna, Microtox TM , and tests using animal ...
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